Order Based on an automated plasmid preparation platform, BiOligo can provide high-quality plasmid DNA preparation services for customers, ensuring one-time extraction of more than 10 μg of high-quality plasmid from different raw materials. The extracted plasmid is free of proteins, as well as residual genome DNA and RNA pollution; the transfection-level DNA can meet requirements on lower endotoxin concentrations.
Service Details
Molecular biology plasmid | Transfection grade plasmid | High-throughput plasmid DNA | |
Volume | μg-g | μg-g | Above 10 μg |
Endotoxin | -- | Quantitative LAL assay ≤0.01 EU/μg DNA | Quantitative LAL assay ≤0.01 EU/μg DNA |
Price | Low | High | High |
Homogeneity | ≥50%±10% supercoiled | ≥90±10% supercoiled | ≥90±10% supercoiled |
Residual RNA | Not visible on agarose gel | ||
Genetic DNA detection | Not visible on agarose gel | ||
Appearance | Colorless, clear, free of precipitate or foreign particles | ||
Sequencing verification | Completely correct | ||
Restriction analysis | DNA bands with expected sizes detected by agarose gel electrophoresis | ||
OD 260/280 | 1.8-2.0 | ||
Concentration | On request, default 1 mg/ml | ||
Bio-Burden Test | No viable microorganisms in /on culture media | ||
Synthesis requirements
1. ≥1 μg plasmid with sufficient purity, a clear electrophoretogram and relevant sequencing information;
2. The provided bacterial solution should be sufficiently active and delivered as specified;
3. The antibiotics and working concentration should be provided if the plasmid is non-ampicillin penicillin, kanamycin or chloramphenicol resistant.
Experimental process
Delivery standards
1. Deliveries of plasmid made based on customer requirements;
2. Puncture fungus or glycerol fungus carrying recombinant plasmid (please specify demands before ordering);
3. QC document: COA document, sequence contrast document, sequencing atlas and plasmid profile.
