Leveraging an automated plasmid preparation platform, BiOligo provides high-quality plasmid DNA preparation services. We ensure the one-time extraction of over 10 μg of high-quality plasmid from various raw materials. The extracted plasmids are free from protein, residual genomic DNA, and RNA contamination, with transfection-grade DNA meeting low endotoxin concentration requirements.
Service Details
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Molecular biology plasmid |
Transfection-grade plasmid |
High-throughput plasmid DNA |
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Delivery quantity |
μg-g |
μg-g |
Above 10 μg |
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Endotoxin analysis |
-- |
≤0.01 EU/μg DNA |
≤0.01 EU/μg DNA |
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Price |
Low |
High |
High |
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Superhelix degree |
≥50% |
≥90% |
≥90% |
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Residual RNA |
Invisible by electrophoresis |
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Genetic DNA detection |
Invisible by electrophoresis |
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Appearance |
Transparent, no obvious particles |
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Sequencing verification |
Consistent with expectations, completely correct |
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Restriction enzyme analysis |
Consistent with the expected size |
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OD 260/280 |
1.8-2.0 |
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Concentration |
The default 1 μg / uL, can be adjusted according to user requirements |
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Synthesis Requirements
- ≥1 μg plasmid with sufficient purity, clear electrophoretogram and relevant sequencing information
- The bacterial solution provided should be sufficiently active and delivered as required
- The antibiotics and working concentration should be provided if the plasmid is non-ampicillin penicillin, kanamycin or chloramphenicol resistant
Experimental Process
Delivery Standards
- Deliveries made based on total customer requirements;
- Puncture bacterium or glycerol strain containing recombinant plasmid (please specify demands before ordering);
- QC document: COA document, sequence alignment document, sequencing graph and plasmid profile.
