Based on an automated plasmid preparation platform, BiOligo can provide high-quality plasmid DNA preparation services for customers, ensuring one-time extraction of more than 10 μg of high-quality plasmid from different raw materials. The extracted plasmid is free of proteins, as well as residual genome DNA and RNA pollution, the transfection-level DNA can meet requirements for low endotoxin concentrations.
Service Details
Molecular biology plasmid | Transfection-grade plasmid | High-throughput plasmid DNA | |
Delivery quantity | μg-g | μg-g | Above 10 μg |
Endotoxin analysis | -- | ≤0.01 EU/μg DNA | ≤0.01 EU/μg DNA |
Price | Low | High | High |
Superhelix degree | ≥50% | ≥90% | ≥90% |
Residual RNA | Invisible by electrophoresis | ||
Genetic DNA detection | Invisible by electrophoresis | ||
Appearance | Transparent, no obvious particles | ||
Sequencing verification | Consistent with expectations, completely correct | ||
Restriction enzyme analysis | Consistent with the expected size | ||
OD 260/280 | 1.8-2.0 | ||
Concentration | The default 1 μg / uL, can be adjusted according to user requirements | ||
Synthesis Requirements
1. ≥1 μg plasmid with sufficient purity, clear electrophoretogram and relevant sequencing information;
2. The bacterial solution provided should be sufficiently active and delivered as required;
3. The antibiotics and working concentration should be provided if the plasmid is non-ampicillin penicillin, kanamycin or chloramphenicol resistant.
Experimental Process
Delivery Standards
1. Deliveries made based on total customer requirements;
2. Puncture bacterium or glycerol strain containing recombinant plasmid (please specify demands before ordering);
3. QC document: COA document, sequence alignment document, sequencing graph and plasmid profile.
