Plasmid DNA preparation

Leveraging an automated plasmid preparation platform, BiOligo provides high-quality plasmid DNA preparation services. We ensure the one-time extraction of over 10 μg of high-quality plasmid from various raw materials. The extracted plasmids are free from protein, residual genomic DNA, and RNA contamination, with transfection-grade DNA meeting low endotoxin concentration requirements.

Service Details

 

Molecular biology plasmid

Transfection-grade plasmid

High-throughput plasmid DNA

Delivery quantity

μg-g

μg-g

Above 10 μg

Endotoxin analysis

 --

≤0.01   EU/μg DNA

≤0.01   EU/μg DNA

Price

Low

High

High

Superhelix degree

≥50%

≥90%

≥90%

Residual RNA

Invisible by electrophoresis

Genetic DNA detection

Invisible   by electrophoresis

Appearance

Transparent, no obvious particles

Sequencing verification

Consistent   with expectations, completely correct

Restriction enzyme analysis

Consistent with the expected size

OD 260/280

1.8-2.0

Concentration

The default 1 μg / uL, can be adjusted according to user requirements

Synthesis Requirements

  •  ≥1 μg plasmid with sufficient purity, clear electrophoretogram and relevant sequencing information
  •  The bacterial solution provided should be sufficiently active and delivered as required
  •  The antibiotics and working concentration should be provided if the plasmid is non-ampicillin penicillin, kanamycin or chloramphenicol resistant

Experimental Process

Delivery Standards

  •  Deliveries made based on total customer requirements;
  •  Puncture bacterium or glycerol strain containing recombinant plasmid (please specify demands before ordering);
  •  QC document: COA document, sequence alignment document, sequencing graph and plasmid profile.