Order Fluorescence quantitative PCR (qPCR) is a PCR detection technique that adds fluorescent groups to the PCR system, monitors the change of fluorescence signal intensity in the PCR reaction system in real time during amplification, and quantifies the target gene by a standard curve. Compared with traditional PCR, fluorescent qPCR technology has high sensitivity, high specificity and high accuracy, and is widely used in various fields of clinical and life science research, such as differential gene expression analysis, SNP detection, and clinical diagnosis of drug development.
BiOligo qPCR technology platform can design and optimize qPCR related raw materials according to the customer's needs. Customers provide specific requirements on qPCR raw material optimization, and BiOligo provides technical optimization services for qPCR assay raw materials and assay solutions.
Service Details
Services project | Details | |
Raw materials for nucleic acid-based detection of Poultry disease and technical services for assay development | AIV, FAd V, ALV, etc. | |
Raw materials for nucleic acid-based detection of Aquatic pathogens and technical services for assay development | ICH, EHP , WSV, etc. | |
Raw materials for nucleic acid-based detection of Pet genetic disease and technical services for assay development | ABCB1, LTBP2, TP0 and other pet-related genes. | |
Raw materials for nucleic acid-based detection of Animal-derived nucleic acid and technical services for assay development | Nucleic acids of animal-derived components such as swine, cows, chickens, cats, dogs, etc. | |
Raw materials for nucleic acid-based detection of human gene and technical services for assay development | Folate gene, psychotropic medication-related gene, alcohol metabolizing ability gene, etc. | |
Raw materials for other customized molecular assays and technical services for assay development | Other customized services such as qPCR primer probe, reaction system design and optimization | |
Service Requirements
Technical service requirements for qPCR raw material optimization and assay development, such as primer and probe optimization to achieve the expected fluorescence increment, reaction system optimization to achieve the desired sensitivity, and amplification condition optimization for better amplification efficiency.
Delivery Information
Provide the validation report of QPCR related raw material design and optimization service, including the specific optimized scheme of raw materials, reaction system, etc., as well as the validation data after the optimized scheme. Validation data include experimental materials,instruments, qPCR systems, qPCR amplification procedures, and experimental results.
