Antisense oligonucleotide (ASO) is a single-stranded DNA or hybrid oligonucleotide, usually 15-25 bases long, which can hybridize with the target RNA sequence by its base sequence arrangement to mediate the cleavage of RNA by RNase H, thereby inhibiting the function of non-coding RNA (such as miRNA, siRNA, piRNA, snoRNA, snRNA, exRNA, scaRNA and lncRNA) or blocking the protein translation of mRNA. In order to improve nuclease resistance, the introduction of phosphorothioate (Phosphorothioate, PS) bonds in the backbone sequence can be used to improve nuclease resistance, thereby enhancing intracellular stability. It has the ability to interfere with both cytoplasmic and nuclear RNA, and can be directly used in in vivo experiments.

Advantages

1. The sequence design is highly specific and can interfere with multiple genes;

2. It has the ability to enter the nucleus, has been chemically modified, has high stability, and has a long effect time in vivo;

3. After special labeling, it can be directly used in in vivo experiments, and can be used with delivery transfection reagents or used alone;

4. Compared with viruses and other carriers, the synthesis cycle is short, the ingredient process is highly controllable, and it is suitable for subsequent nucleic acid drug research and development.

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BLG-siASO is a hybrid form of siRNA and ASO, which can simultaneously inhibit the effective interference of target lncRNA, mRNA or circRNA in the nucleus or outside the nucleus.