IVT RNA Production

As a multifunctional biomolecule, RNA is divided into coding RNA and non-coding RNA (ncRNA), where ncRNA includes various types such as tRNA, lncRNA, miRNA, small interfering RNA (siRNA), saRNA, and circRNA

IVT RNA Production solutions streamline the workflow from gene synthesis to in vitro transcriptional RNA production, providing high-quality, providing high-quality and large-scale RNA for RNA research, with production scales from 20 μg to 1 mg levels.

Service details

Type

Order

5’ cap

NTP modification

RNA modification

Ploy A-tail

lncRNA

20 μg-1 mg

N1-Me-pUTP

5-OMe-UTP

m6A-ATP

Pseudo-UTP

5-Me-CTP

3’ Biotin

DIG-11- UTP

Biotin-UTP

Cy5-UTP

No Tail

mRNA

20 μg-1 mg

Cap 0、Cap 1

100A Tail、Support customization

* If there is a need for g level, please consult sales.

Quality Control

IncRNA QC

Quality	Attribute	Method
Identity	Sequence confirmation	Sanger sequencing
Content	RNA concentration	Nanodrop quantification
Purity	A260/280	Nanodrop quantification
Purity	The content of the target band	Grayscale analysis
Integrity	RNA length	Agarose gel electrophoresis

mRNA QC

Quality	Attribute	Method	Research	Clinical
Identity	Sequence confirmation	Sanger sequencing	√	√
Content	RNA concentration	Nanodrop quantification	√	√
Integrity	3’ poly(A)	LC-MS	×	√
	5’ cap	LC-MS	√	√
	mRNA Integrity	Capillary gel electrophoresis	×	√
	mRNA Integrity	Agarose gel electrophoresis	√	√
Purity	Percentage of intact mRNA and fragment mRNA	Grayscale analysis	√	√
Purity	A260/280	Nanodrop quantification	√	√
Impurity	Residual DNA template	qPCR	×	√
Impurity	dsRNA	ELISA	×	√
Safety	Endotoxin	Limulus amebocyte lysate	×	√
	Sterility	Media	×	√
	Bioburden	Microbial enumeration tests	×	√
Other	PH	PH计	√	√
Other	Appearance		√	√