Bioligo offers a comprehensive NGS solution, providing a one-stop service from probe/primer design and synthesis to library preparation, sequencing, and bioinformatics analysis!

In NGS whole-genome sequencing, the large volume of data makes analysis challenging. When sequencing target genes related to specific diseases or tumors, targeted capture of the relevant regions is often chosen. This approach reduces sequencing costs, increases sequencing depth, and improves data efficiency, making it suitable for detecting mutations such as SNPs, InDels, CNVs, and fusions.

There are two main approaches for targeted capture:

1. Multiplex PCR:
   This method uses universal primers to amplify gene fragments, effectively enriching the target sequences. However, the number of amplicons in a single experiment is limited, and the design process can be complex.

2. Hybridization Capture:
   Biotin-labeled probes capture the target gene fragments through base pairing. After hybridization, magnetic beads are used to separate the probe-bound target fragments from non-target sequences, enabling sequencing of the desired fragments. Compared to multiplex PCR, hybridization capture offers several advantages: it can detect a broader range of mutation types, works for panels of various sizes, is more tolerant of mismatches in probe binding, and provides a wider detection scope.

BiOligo offers a comprehensive NGS solution with both multiplex amplicon sequencing and hybridization capture sequencing, featuring the following features:

1. Custom-designed capture probe panels and multiplex PCR primer panels, solving the issue where commercially available panels may not meet project-specific needs.

2. A complete set of reagents for targeted sequencing, addressing the compatibility issues that arise when sourcing reagents from multiple suppliers.

3. Customizable bioinformatics analysis methods and workflows, tailored to customer requirements.