Capture Probe Design

Design Features

1. Multiple Input Formats: Supports BED, FASTA, GeneSymbol, etc.

2. Optimized Algorithm: Balances probe GC content, specificity, dimerization, and secondary structures, maximizing coverage with fewer probes to reduce costs.

3. Flexible Parameters: Choose from density modes (1x, 2x tiling, overlap) and reference genomes, with customizable risk grading for sequence specificity and structural regions.

4. Tailored Design Options: Different algorithms for various regions like gene, intergenic, SNP, and MSI, ensuring optimal design.

5. Sustainability: Add new probes to an existing panel using spike-in, creating a customizable, evolving panel.

6. User-Friendly: SmartBaits uses a relational database backend, minimizing memory load and computation time, with easy reanalysis using multiple criteria.