Site-directed mutagenesis introduces specific variations to a target DNA fragment via PCR, encompassing base additions, deletions, and point mutations. As a common method for gene transformation and optimization, and a powerful tool for investigating the complex relationship between protein structure and function, site-directed mutagenesis can be applied to a wide range of genes. Based on customer-provided mutation requirements, BiOligo can introduce single or multiple base group mutations into a plasmid template, ensuring the accurate acquisition of the DNA clone for direct use in downstream research.

Advantages

• Accurate Sequencing: We guarantee 100% accuracy of the intended mutation, with no unintended changes introduced.

• Target Any Site: Mutations can be precisely introduced at any desired location within the DNA fragment.

• Customized Solutions: We can accommodate complex requests, including mutations involving repeated sequences, inverted repeats (card structures), hairpin structures, and high GC content, all tailored to your specific needs.

• Rapid Synthesis: For common sequences, we ensure delivery within 7 business days.

Service Details

Service Name	Length	Lead Time (Business Day)
Site-directed mutagenesis	<1.5 kb	7~10
	1.5 kb~3 kb	7~11
	3 kb~6 kb	10~15
	6 kb~8 kb	13~18
	>8 kb	Inquire

* Counted as one mutation site within 40 bp;

* Suitable for standard carriers only, service for non-conventional carriers should be negotiated after specific evaluation.

 

Synthesis Requirements

1. Plasmid required for gene mutation (>100 ng/μl) above 20 μl, or fresh glycerin bacterial solution >200 μl;

2. Plasmid information (atlas, sequence, size, resistance, etc.);

3. Position of mutation position and total length of sequence.

 

Experimental Process

 

Delivery Standards

• 3 μg lyophilized plasmid DNA

• Stab culture or glycerol stock carrying recombinant plasmid

• Sequence comparison report

• Sequencing chromatogram report

• Plasmid profile

• COA