Site-directed mutagenesis introduces specific variations to a target DNA fragment via PCR, encompassing base additions, deletions, and point mutations. As a common method for gene transformation and optimization, and a powerful tool for investigating the complex relationship between protein structure and function, site-directed mutagenesis can be applied to a wide range of genes. Based on customer-provided mutation requirements, BiOligo can introduce single or multiple base group mutations into a plasmid template, ensuring the accurate acquisition of the DNA clone for direct use in downstream research.
Advantages
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Accurate Sequencing: We guarantee 100% accuracy of the intended mutation, with no unintended changes introduced.
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Target Any Site: Mutations can be precisely introduced at any desired location within the DNA fragment.
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Customized Solutions: We can accommodate complex requests, including mutations involving repeated sequences, inverted repeats (card structures), hairpin structures, and high GC content, all tailored to your specific needs.
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Rapid Synthesis: For common sequences, we ensure delivery within 7 business days.
Service Details
Service Name |
Length
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Lead Time (Business Day)
|
Site-directed mutagenesis |
<1.5 kb |
7~10 |
1.5 kb~3 kb |
7~11 |
|
3 kb~6 kb |
10~15 |
|
6 kb~8 kb |
13~18 |
|
>8 kb |
Inquire |
* Counted as one mutation site within 40 bp;
* Suitable for standard carriers only, service for non-conventional carriers should be negotiated after specific evaluation.
Synthesis Requirements
1. Plasmid required for gene mutation (>100 ng/μl) above 20 μl, or fresh glycerin bacterial solution >200 μl;
2. Plasmid information (atlas, sequence, size, resistance, etc.);
3. Position of mutation position and total length of sequence.
Experimental Process
Delivery Standards
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3 μg lyophilized plasmid DNA
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Stab culture or glycerol stock carrying recombinant plasmid
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Sequence comparison report
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Sequencing chromatogram report
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Plasmid profile
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COA