By introducing the required variations to target the DNA fragment through PCR based on mutation demands, the site-directed mutation includes base adding, deletion and point mutation. As a common means for transforming and optimizing genes, and as a powerful tool for researching the complex relationship between protein structure and function, the site-directed mutation can be applied to a number of different genes. BiOligo can provide mutations to single or multiple basic groups in a plasmid template, based on the mutation demands provided by the customer, in order to accurately acquire the DNA clone and use it directly for downstream research.

Advantages

1. Accurate sequencing: Ensures 100% accuracy of the mutant and avoids any other mutations.

2. Any point: The mutation can be introduced at any site of the DNA fragment.

3. Customized: Able to provide mutations containing repeated and card structures, hairpin structures and high GC content according to customer requirements.

4. Rapid synthesis: Common sequences can be delivered within 3 working days.

Service Details

* Counted as one mutation site within 40 bp;

* Suitable for standard carriers only, service for non-conventional carriers should be negotiated after specific evaluation.

Synthesis Requirements

1. Plasmid required for gene mutation (>100 ng/μl) above 20 μl, or fresh glycerin bacterial solution >200 μl;

2. Plasmid information (atlas, sequence, size, resistance, etc.);

3. Position of mutation position and total length of sequence.

Experimental Process

Delivery Standards

• 3 μg lyophilized plasmid DNA

• Stab culture or glycerol stock carrying recombinant plasmid

• Sequence comparison report

• Sequencing chromatogram report

• Plasmid profile

• COA