Order Single nucleotide polymorphism (Single Nucleotide Polymorphism, SNP) mainly refers to DNA sequence polymorphism caused by the variation of a single nucleotide at the genome level, which exists in the form of single base inversion, conversion, insertion and deletion. SNPs are widely distributed in animal genomes, and the probability of a single nucleotide mutation is about 10-9. As a third generation of genetic markers, SNPs are widely distributed and play an important role in many fields such as genetic analysis, disease diagnosis, molecular breeding and population ecological evaluation.
BiOligo's professional technology platform ensures accurate genotyping results, and can provide full-process services such as nucleic acid extraction, probe design and verification, detection and result analysis according to customer needs.
Service Details
S/N | Method | Introduction | Period |
BLG-S01 | Sanger sequencing | DNA extraction, SNP design, etc. | 1 week |
BLG-S02 | Taqman probe | Primer design for different SNP points on the chromosome and qPCR amplification of probe | 2-3 weeks |
BLG-S03 | Snapshot | Synthetic primer design, DNA quality inspection, PCR, extension, sequencing instrument detection and analysis report | 4-8 weeks |
BLG-S04 | Mass spectrometry | Synthetic primer design, DNA quality inspection, PCR, extension, Sequenom mass spectrometer detection and analysis report | 4-6 weeks |
Synthesis Requirements
1. Cell sample: Cell number ≥106, cell suspension or cell pellet;
2. Tissue sample: Tissue dose is over 100 mg (sized soybean);
3. Blood sample: EDTA anti-freezing agent is required in the sample, total volume ≥500 μl;
4. FFPE sample
5. DNA: Volume ≥10 μl, concentration ≥50 ng/μl, clear strip and free of obvious degradation;
6. PCR product: Concentration ≥20 ng/μl, volume ≥10 μl.
Experimental Process
Direct sequencing method
* SNP experimental design includes target selection and primer design.
Taqman probe method
* SNP experimental design includes target selection and primer/probe pair design.
Delivery Criteria
Provide a detailed project report, including raw data, sequencing results, collated analysis data and genotyping results.
