Multiplex PCR (mPCR) is a nucleic acid detection technology that simultaneously amplifies multiple target sequences in a single PCR reaction. Since its introduction in 1988, mPCR has become widely used in scientific research and disease diagnostics due to its efficiency, high throughput, and low cost.

Although mPCR allows for rapid and cost-effective detection of multiple targets, it also faces challenges such as primer design, optimization of reaction conditions, non-specific amplification, and primer dimer formation. Careful optimization of primer specificity, length, GC content, and annealing temperature is crucial to ensure balanced amplification of target fragments.

Multibaits improves primer design by generating a large number of primer sequences and selecting quality primers based on multiple criteria. The latest dimer theory and annealing optimization algorithms are then used to achieve optimal primer combinations.

Process Overview

The process consists of three main stages: primer generation, primer selection, and primer combination.

Product Features

1. Professional design tools for optimal multiplex primer design.

2. Low-base error rate synthesis process ensures high amplification efficiency and low non-specific amplification.

3. Automated primer quantification, aliquoting, and mixing for accurate and uniform distribution.

4. Amplification system supports up to 7,000 targets.

5. Strategies are provided to address challenging sites, improving overall site coverage.