qPCR Probes

Quantitative PCR (qPCR), also known as real-time PCR, is an essential tool in fundamental research and clinical diagnostics. It plays a vital role in detecting infectious diseases, cancer biomarkers, genetic disorders and other targets.

BiOligo provides customized qPCR probes, including TaqMan probes, molecular beacon probes and super-quenched probes, all tailored to meet your specific research needs.

 

Probe Design

The selections of fluorophores and quenchers are essential to probe design:

  • An appropriate fluorophore choice is related to the qPCR instrument used;
  • An appropriate quencher choice is related to the quenching mechanism involved.
Probe type Melting curve probe Super quench Taqman Molecular beacon
RQ SQ BHQ BHQ-X TAMRA Eclipse Dabcyl
/ 430- NIR 430 nm- NIR 390-625 nm 520-600 nm 390-625 nm 380-530 nm
Tm Enhancement N N N Increasing by 10 degrees or more N N N
A-T Rich Sequence Compatibility Y Y N Y N N N
Background fluorescence None None None None Yes None None
Sensitivity +++ +++++ +++ ++++ ++ +++ +
Specificity +++ +++ + +++ + + ++
Design Complexity + + + ++ + + +++
Synthesis Complexity + + + +++ + + ++

 

 

Common Fluorophore and Quencher Combinations

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