qPCR Probes
Quantitative PCR (qPCR), also known as real-time PCR, is an essential tool in fundamental research and clinical diagnostics. It plays a vital role in detecting infectious diseases, cancer biomarkers, genetic disorders and other targets.
BiOligo provides customized qPCR probes, including TaqMan probes, molecular beacon probes and super-quenched probes, all tailored to meet your specific research needs.
Probe Design
The selections of fluorophores and quenchers are essential to probe design:
- An appropriate fluorophore choice is related to the qPCR instrument used;
- An appropriate quencher choice is related to the quenching mechanism involved.
| Probe type | Melting curve probe | Super quench | Taqman | Molecular beacon | |||
| RQ | SQ | BHQ | BHQ-X | TAMRA | Eclipse | Dabcyl | |
| / | 430- NIR | 430 nm- NIR | 390-625 nm | 520-600 nm | 390-625 nm | 380-530 nm | |
| Tm Enhancement | N | N | N | Increasing by 10 degrees or more | N | N | N |
| A-T Rich Sequence Compatibility | Y | Y | N | Y | N | N | N |
| Background fluorescence | None | None | None | None | Yes | None | None |
| Sensitivity | +++ | +++++ | +++ | ++++ | ++ | +++ | + |
| Specificity | +++ | +++ | + | +++ | + | + | ++ |
| Design Complexity | + | + | + | ++ | + | + | +++ |
| Synthesis Complexity | + | + | + | +++ | + | + | ++ |
Common Fluorophore and Quencher Combinations
