qPCR Probe

Quantitative PCR (qPCR), also known as real-time PCR, is an essential tool in fundamental research and clinical diagnostics. It plays a vital role in detecting infectious diseases, cancer biomarkers, genetic disorders and other targets.

BiOligo provides customized qPCR probes, including TaqMan probes, molecular beacon probes and super-quenched probes, all tailored to meet your specific research needs.



Probe Design

The selections of fluorophores and quenchers are essential to probe design:

a. an appropriate fluorophore choice is related to the qPCR instrument used;

b. an appropriate quencher choice is related to the quenching mechanism involved.


Probe typeMelting curve probeSuper quenchTaqmanMolecular beacon
RQ(SQ)(BHQ)(BHQ-X )(TAMRA)(Eclipse)(Dabcyl)
/430- NIR430 nm- NIR390-625 nm520-600 nm390-625 nm380-530 nm
Tm EnhancementNNNIncreasing by 10 degrees or moreNNN
A-T Rich Sequence CompatibilityYYNYNNN
Background fluorescenceNoneNoneNoneNoneYesNoneNone
Sensitivity+++++++++++++++++++++
Specificity++++++++++++++
Design Complexity++++++++++
Synthesis Complexity++++++++++




Common Fluorophore and Quencher Combinations

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