Product Details

The kit adopts common alkaline lysis method and absorbs target plasmids based on the specificity of the High-yield DNA absorption column to complete plasmid extraction in 25 minutes, which applies to 4-20 ml fresh E. coli culture medium, with the max. absorption amount of 100 μg plasmids, with the endotoxin content ≤0.1 EU/μg; the quality of the plasmid extraction is related to the copy number of plasmids, host bacteria, the way of lysis and bacterial concentration; the extracted target plasmid applies to routine molecular cloning experiments, including enzyme digestion, PCR, DNA sequencing and transfection experiments on sensitive cells.

Taking plasmid pUC57 as example, if cultivation is made via our developed plasmid fermentation medium (Cat No. SDM0101) without animal-derived components, 70-100 μg plasmid can be extracted from 10 ml bacterial medium; the yield of plasmid is 2-3 times higher than that of conventional LB culture medium.

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