400-168-8906
qPCR Probe Order

Advantages


1.   Stable quality:For the same customer, using the same process parameters and quality control system for research and production orders ensures a smooth transition from research to production, avoiding the need for secondary development during production transfer.

2.   Minimal batch-to-batch variation: Large-scale synthesis and purification can reach up to 300,000 OD in a single run, completing production orders in one batch with minimal intra-batch variation.

3.   Efficient synthesis:Utilizing lean Six Sigma management methods, adhering to ISO9001 and ISO13485 quality control requirements, and combining excellent process flow and technical management systems to ensure a high success rate on the first attempt.

4.   Accurate quantification:Using precise conversion between nmol and OD, employing measurement system repeatability and reproducibility (Gage R&R) validation methods to ensure the stability of the quantification system.

5.   Comprehensive quality inspection reports: Including MASS, HPLC, full-wavelength scanning, quantification, fluorescence intensity detection, etc.

6.   Well-documented process records: Ensuring good traceability of core raw materials' quality.




Probe type

 

Probe

Quencher

Quenched Range

Taqman probe

BHQ series

430 nm- NIR

BHQ-X

390-625 nm

TAMRA

520-600 nm

Eclipse

390-625 nm

Molecular beacon probe

Dabcyl

380-530 nm

Super-quenched probe

SQ

430-650 nm



Probe selection

 

Probe Type

Super-quenched

Taqman

Molecular Beacon

(SQ)

(BHQ)

(BHQ-X)

(TAMRA)

(Eclipse)

(Dabcyl)

Increase primer

Tm value

N

N

Improved >10℃

N

N

N

Applicable for

 A-T rich

Y

N

Y

N

N

N

Background   fluorescence

N/A

N/A

N/A

Available

N/A

N/A

Sensitivity

+++++

+++

++++

++

+++

+

Specificity

+++

+

+++

+

+

++

Design difficulty

+

+

++

+

+

+++

Difficulty of chemical   synthesis

+

+

+++

+

+

++