Advantages
1. Stable quality:For the same customer, using the same process parameters and quality control system for research and production orders ensures a smooth transition from research to production, avoiding the need for secondary development during production transfer.
2. Minimal batch-to-batch variation: Large-scale synthesis and purification can reach up to 300,000 OD in a single run, completing production orders in one batch with minimal intra-batch variation.
3. Efficient synthesis:Utilizing lean Six Sigma management methods, adhering to ISO9001 and ISO13485 quality control requirements, and combining excellent process flow and technical management systems to ensure a high success rate on the first attempt.
4. Accurate quantification:Using precise conversion between nmol and OD, employing measurement system repeatability and reproducibility (Gage R&R) validation methods to ensure the stability of the quantification system.
5. Comprehensive quality inspection reports: Including MASS, HPLC, full-wavelength scanning, quantification, fluorescence intensity detection, etc.
6. Well-documented process records: Ensuring good traceability of core raw materials' quality.
Probe type
Probe | Quencher | Quenched Range |
Taqman probe | BHQ series | 430 nm- NIR |
BHQ-X | 390-625 nm | |
TAMRA | 520-600 nm | |
Eclipse | 390-625 nm | |
Molecular beacon probe | Dabcyl | 380-530 nm |
Super-quenched probe | SQ | 430-650 nm |
Probe selection
Probe Type | Super-quenched | Taqman | Molecular Beacon | ||||
(SQ) | (BHQ) | (BHQ-X) | (TAMRA) | (Eclipse) | (Dabcyl) | ||
Increase primer Tm value | N | N | Improved >10℃ | N | N | N | |
Applicable for A-T rich | Y | N | Y | N | N | N | |
Background fluorescence | N/A | N/A | N/A | Available | N/A | N/A | |
Sensitivity | +++++ | +++ | ++++ | ++ | +++ | + | |
Specificity | +++ | + | +++ | + | + | ++ | |
Design difficulty | + | + | ++ | + | + | +++ | |
Difficulty of chemical synthesis | + | + | +++ | + | + | ++ |