Order BiOligo is equipped with self-developed high-throughput DNA synthesizers, advanced ammonium deprotection devices, and high-precision automated workstations. Utilizing intelligent monitoring methods, BiOligo ensures online monitoring of the production of each primer, strict quality control, and high-quality output. BiOligo is dedicated to providing customers with large-scale, high-quality primer synthesis services.
Services
Conventional primers | Modified primers | Oligo Pools | STR primers | MALDI-TOF primers | Others |
Advantages
1. Prevent external contamination and cross-contamination
2. Ensure consistency between synthesis batches
3. Address quality fluctuations across different specifications
4. Multiple options for modifications
5. Timely delivery and stable supply
Custom Synthesis
Purification Method
Purification | Method | Purity | |
DSL | C18 column desalination | >70% | Advantages: High throughput, short period, low cost; Disadvantages: Difficult to remove shorter fragments; Application: Suitable for short-chain or non-high purity primers. |
UPAGE | OPC column purification | >80% | Advantages: High throughput, short period, high cost performance; Disadvantages: Efficiency to remove fragments above 45 nt steadily decreases; Suggestion: Suitable for primers with fewer than 45 basic groups. |
PAGE | Polyacrylamide gel electrophoresis | >90% | Advantages: Removes synthesized short-chain primers, good effect for long-chain primers (>45 nt) or primers with high quality requirements, with purity over 90%; Disadvantages: Low capacity, complex operation. |
HPLC | High performance liquid chromatography | >95% | Advantages: High purity (over 95%) and sensitivity; Disadvantages: High cost; Suggestions: Suitable for primers with fewer than 40 basic groups, good purification effect for primers with special modifications. |
DHPLC | Double purification combination | >98% | Suggestion: Suitable for primers and probes with special requirements. |
Synthesis Unit
Provide synthesis quantity options based on customer requirements in units of OD or nmol..
