Product Details

The plasmid or protein extracted from E.coli cells is usually contaminated by endotoxin, which can bind with the receptors on cell membrane to cause immune reactions. The endotoxin also has strong toxic effect, which can affect the growth and function of the cells. Therefore, endotoxin-free biological products (less than 0.1 EU/ml or even lower) are required in immunology experiments, cell culture experiments, cell therapy experiments and cell apoptosis experiments. At present, the conventional methods for removing endotoxin include ion-exchange adsorption, affinity-filtration adsorption, ultrafiltration etc. But these methods are tedious and time-consuming ,with high requirements on equipment.

The product can specifically bind with the endotoxin in DNA, recombinant protein and other liquid samples. After ice bath and heat incubation, DNA or protein can be kept in the water phase under high-speed centrifugation, while the endotoxin is concentrated in the lower phase. The product is suitable for endotoxin removal experiments in DNA, protein or other liquid samples. After three removal experiments or more, the endotoxin content can be reduced by 1000-10000 times, from 5000-50000 EU/ml to 5-0.5 EU /ml. The deceloped endotoxin removal reagent  is efficient and fast, with low requirements on equipment.

Advantages

High endotoxin removal efficiency, after more than 3 repeated extraction can reduce the level of endotoxin 1000-10000 times; Low requirements for equipment.

Component

Downloadable files