Uracil-N-glycosylase (UNG) is frequently used to eliminate residual PCR contamination. In this method, dUTP is incorporated into PCR amplification products by either mixing it with dNTPs at a specific ratio or substituting it entirely for dTTP. To remove carry-over contamination from previous PCR reactions, simply add 0.2-1 unit of UNG to the PCR mix and include a 2-10 minute incubation step at 37°C before initiating PCR amplification. UNG is then conveniently inactivated during the pre-denaturation step (2 minutes at 95°C) within the PCR cycle.

BiOligo provides both E. coli UNG and Heat-labile UNG to fully meet the needs of your diverse experiments.