This complex guides the nuclease Cas9 protein to cut double-stranded DNA at the sequence target site paired with the crRNA. The two RNAs, crRNA and tracrRNA, can be modified to form a guiding gRNA that directs Cas9 to targeted DNA cleavage. BiOligo tracrRNA synthesis service can rapidly exert gene editing effects, reduce off-target rates, and improve transfection efficiency to better meet your experimental needs.