Product Details

The kit adopts common alkaline lysis method and absorbs target plasmids based on the specificity of the DNA absorption column to complete plasmid extraction, which applies to 1-5 ml fresh E. coli culture medium, with the max. absorption amount of 30 μg plasmids, with the endotoxin content < 0.1 EU/μg; the quality of the plasmid extraction is related to the copy number of plasmids, host bacteria, the way of lysis and bacterial concentration; the extracted target plasmid applies to routine molecular cloning experiments, including digestion, PCR, DNA sequencing and transfection experiments on sensitive cell.

Advantages

High extraction efficiency, taking plasmid pUC57 as example,  if cultivation is made via our developed plasmid fermentation medium (Cat No. SDM0101) without animal-derived  components, 15-20 μg plasmid can be extracted from 2 ml bacterial medium; the yield of plasmid is 2-3 times higher than that of conventional LB culture medium.

Component

Downloadable files