Product Details

The T7 High Yield RNA Synthesis Kit optimizes the transcription reaction system. The T7 RNA polymerase uses linear double stranded DNA containing the T7 promoter as a template and NTP as a substrate to transcribe the DNA sequence downstream of the promoter. Easy and fast access to large amounts of RNA.The T7 High Yield RNA Synthesis Kit can be used to transcribe unlabeled RNA, with a 0.1-1 ug template, producing 150-200 ug of RNA. The RNA synthesized by transcription can be used for downstream studies such as RNA structure and function studies, RNA enzyme protection, probe hybridization, RNAi, microinjection, and in vitro translation.

Advantages

• Enhanced Synthesis Efficiency

Our optimized T7 RNA polymerase significantly boosts RNA synthesis efficiency.

• Flexible Template Input

The method accommodates a wide range of template inputs, suitable for various sample preparations.

• High Yield

Each standard reaction yields up to 200 μg of RNA, with in vitro transcribed RNA that is suitable for a wide variety of downstream applications.

• Streamlined Workflow

The kit includes an NTP Mix, which reduces hands-on time and minimizes the risk of contamination.

 

Product Data

Using four different DNA templates, the BiOligo T7 RNA In Vitro Transcription Kit successfully produced RNA transcripts of corresponding sizes. The reaction was completed with a two-hour incubation at 37°C.

Component

Downloadable files