Product Details

The assay is based on the TaqMan principle and relies on the 5’→3’ exonuclease activity of Taq polymerase as well as the dual labeling of the probes with fluorescents and quenchers. During PCR the Taq polymerase cleaves and removes annealed probes, releasing the previously quenched fluorescent signal.

The target (Pork/Rat/Dog) sequence is a mitochondrial multi-copy gene. The assay is composed of two principal steps: extraction of total DNA from meat (or food) samples, and the BioZues® HALAL Real-time PCR Kit (Pork/Rat/Dog), a qualitative duplex real-time PCR test for the detection of Pork/Rat/Dog-specific genes and exogenous internal positive standards (IC) using specific primers and probes labeled with fluorescent dyes. The target sequences and IC are detected through the FAM and HEX (VIC) change,l respectively.

Advantages

• High Sensitivity

Limit of detection: 0.0002% of target DNA

• High Specificity

Get accurate results even in complex food samples. Our assay is designed with no cross-reactivity to beef, lamb, chicken, duck, or goose DNA, ensuring reliable and specific detection in mixed food backgrounds.

• Reliable Quality Control

The kit includes a synthetic Internal Positive Control (IPC) to monitor DNA extraction efficiency and detect PCR inhibitors, which minimizes the risk of false-negative results.

 

Product Data

• High Sensitivity

The kit demonstrates high sensitivity, with a detection limit of 0.0002% (2ppm, parts per million) achieved in both singleplex and triplex assays.

Fig 1. Simultaneous detection of pork, dog, and rat DNA targets in a mixed-template format.

Pork （CY5 channel）	Dog （ROX channel）	Rat （FAM channel）	Pork & Dog & Rat

Nucleic Acid Concentration (pg/μL)	200	20	2	0.2	0.02
Pork Average Cт	17.02	20.29	25.05	29.86	33.39
Dog Average Cт	21.27	24.68	28.09	31.76	33.58
Rat Average Cт	16.57	20.04	23.37	28.37	30.31

• High Specificity
  The kit maintains accurate detection of pork, dog, and rat DNA targets, even in the presence of 50 ng bovine DNA, and ensures accurate results in complex food backgrounds.
  Fig. 2. Detection of pork, dog, and rat DNA targets in the presence of 50 ng bovine DNA.

  Pork （CY5 channel）	Dog （ROX channel）	Rat （FAM channel）	Pork & Dog & Rat

  Nucleic Acid Type	Species	Nucleic Acid Concentration (pg/μL)	Average Cт	CV (%)
  mixture of three target DNAs (pork, dog, rat) + bovine DNA	Pork	0.02	26.95	0.37%
  	Dog	0.02	31.06	3.80%
  	Rat	0.02	26.65	0.87%
  	Internal Control	100,000	24.88	1.07%

Component

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