Product Details
BecuNGS® Fast DNA Library Prep Kit for Illumina is a new generation enzyme-digested library preparation kit for the Illumina high-throughput sequencing platform. This kit combines DNA fragmentation, end repair, and dA tailing into one step. The fragmentation product does not need to be purified, and can be directly ligated with adapters. It can convert 100 pg-1 μg template DNA into a special library for Illumina high-throughput sequencing platform, suitable for Construction of PCR and PCR-Free libraries.
This kit is perfectly compatible with DNA from different sources and different input amounts, and you only need to adjust the fragmentation time according to the size of the target insert to get the required fragment size Libraries can be used for animal whole blood DNA, animal and plant tissue DNA, cell DNA and other samples. This product can be applied to whole genome sequencing, whole exome or other targeted capture sequencing, and metagenomic sequencing.
Product data
Broad DNA Input Range
Using the NA12878 standard, library construction with inputs ranging from 1000 ng (2 PCR cycles) down to 0.1 ng (16 cycles) and 10 minutes of enzymatic fragmentation consistently produced libraries with well-controlled sizes and clear main peaks.
Adjusting Main Peak Size Based on Enzymatic Digestion Time
Using 250 ng of NA12878 standard with fragmentation times of 5, 10, and 20 minutes, we consistently produced libraries with clear, distinct peak profiles, demonstrating that the main peak size is a direct function of the fragmentation duration.
Compatible with Various Sample Types
Libraries were successfully constructed using 250 ng of NA12878 standard DNA, mildly and moderately degraded FFPE DNA, and whole blood gDNA. With just 10 minutes of enzymatic fragmentation and 5 PCR cycles, all samples yielded sharp, concentrated library peaks with no residual smearing.
Advantages
Easy to use, this kit combines DNA fragmentation, end repair, and dA tailing into one step, the fragmentation product does not need to be purified, and can be directly ligated with adapters; strong compatibility: compatible with DNA from different sources and different input amounts.
Component
Downloadable files
BecuNGS® Fast DNA Library Prep Kit for illumina