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Super-quenched Probes

To reduce background fluorescence caused by incomplete quenching in traditional TaqMan probes, BiOligo developed the Super-Quenched (SQ) probe. By adding an extra internal quencher and a unique 3' quencher, this design effectively lowers background noise, improves sensitivity, and prevents fluorescence leakage.

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Features

  • High Sensitivity | LOD to 1 copy

  • High Signal-to-Noise Ratio (SNR) | Effectively reduces background fluorescence, increase endpoint fluorescence signal intensity

  • Ideal for Multiple qPCR Detection | Minimizes fluorescence crosstalk, minimize non-specific amplification

  • Applicable to AT-rich Targets

Recommended Combinations

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Cases

Comparison of Modified Fluorescence for Different Quenchers in the Same Sequence.

qPCR Applicable —— Lower background signal and higher SNR

Channel
Target
 Figure
FAM
AsFV-p72
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HEX
Monkeypox plasmid
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Cy5
COVID-19
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