BiOligo offers customized synthesis of mass spectrometry primers for clients based on their specific requirements. Single nucleotide polymorphism (SNP) detection, performed using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF), achieves an accuracy rate of up to 99.9%. With high accuracy, flexibility, high throughput, short detection cycles, and cost-effectiveness, it provides an excellent solution for SNP detection.

Advantages

• Specialized synthesis and purification processes are employed to achieve primer purity≥95%.
• Strict control of N+1/N-1 fragment ratio to avoid false positives during detection.
• Proprietary purification processes are employed to minimize salt impurities in the primer, avoiding the introduction of salt peaks in mass spectrometry results that could interfere with interpretation.
• Accurate quantification with an error rate ≤ ±10%, avoiding non-specific amplification during multiple PCR processes.

Experimental Procedure

• PCR amplification of DNA sequences containing the target SNP, followed by purification to remove unbound dNTPs.
• iPLEX single nucleotide extension: Purified PCR products are used for single nucleotide extension, with a specific extension primer designed for each target SNP. The extension products for each SNP allele differ by only one base at the end.
• Mass spectrometry detection: Purified extension products are transferred to a SpectroCHIP chip for mass spectrometry analysis. Different alleles of the same SNP are distinguished by separate detection peaks resulted from their distinct molecular weights.