BiOligo offers custom synthesis of mass spectrometry primers tailored to client requirements. SNP detection using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry can achieve up to 99.9% accuracy. With high precision, flexibility, high throughput, short detection cycles, and cost-effectiveness, this solution provides an excellent platform for SNP analysis.

Advantages

• Specialized synthesis and purification processes are employed to achieve primer purity≥95%.
• Strict control of N+1/N-1 fragment ratio to avoid false positives during detection.
• Proprietary purification minimizes salt impurities, avoiding salt peaks that could interfere with mass spectrometry interpretation.
• Accurate quantification with ≤ ±10% error prevents non-specific amplification in multiplex PCR.

Experimental Procedure

• PCR Amplification: DNA sequences containing the target SNP are amplified by PCR, followed by purification to remove residual dNTPs.

• iPLEX Single Nucleotide Extension: Purified PCR products undergo single-nucleotide extension using a specific extension primer designed for each target SNP. The extension products for each SNP allele differ by a single nucleotide at the terminal position.

• Mass Spectrometry Detection: Purified extension products are transferred to a SpectroCHIP for mass spectrometry analysis. Different alleles of the same SNP are distinguished by separate detection peaks resulting from their distinct molecular weights.