Regular crRNA

Next Generation Sequencing
- Library Preparation
  - Stocked Adapters
    - Full-length Adapters
    - Truncated Adapters
    - UMI Adapters
  - Library Preparation Kits
    - DNA Library Prep Kits
      - Library Prep Modules
    - RNA Library Prep Kits
    - DNA&RNA Library Prep Kits
    - Methyl-seq Library Prep Kits
- Hybridization Capture
  - Stocked Blockers
  - Stocked Panel
  - Hybridization Capture Kit
  - Hybridization Capture Reagents
- Magnetic Bead

RNAi & CRISPR
- RNAi
  - siRNA
  - miRNA
- CRISPR
  - gRNA
  - crRNA
  - tracrRNA

qPCR/PCR Kits & Reagents
- PCR
  - Taq DNA Polymerase
  - Hot-Start DNA Polymerase
  - PCR Mix
- qPCR
  - qPCR Mix
    - SYBR Green qPCR
    - Probe-based qPCR
  - qPCR Assay
    - Calibration Fluorescent Probe
    - Endogenous Control Probes
    - Free Dyes for Calibration
    - PCR/RT-PCR Kits
    - Dye-labeled Calibrating Oligos
- RT-PCR
  - Reverse Transcription
  - One Step RT-qPCR Kit
- Other Enzymes
  - RNase Inhibitor
  - Uracil N-Glycosylase (UNG)
    - UNG
    - Heat-labile UNG
  - T4 DNA Polymerase

Other Lab Supplies
- In vitro Transcription
- Nucleic Acid Extraction
  - Plasmid Extraction Kit
- Transfection Reagent
- Gel Electrophoresis
  - DNA Ladders
  - Loading Buffer
  - Agarose

Regular crRNA

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Product Details

crRNA (CRISPR-derived RNA) binds to tracrRNA through base pairing to form the tracrRNA/crRNA complex, which guides the nuclease Cas9 protein to cut double-stranded DNA at the sequence target site paired with crRNA. The two RNAs, crRNA and tracrRNA, can be artificially engineered to form a guide gRNA that directs Cas9 to targeted DNA cleavage. The BiOligo crRNA synthesis service can rapidly exert gene editing effects, reduce off-target rates, and improve transfection efficiency to better meet your experimental needs.

Service Details

crRNA Service	Characteristics	Application
Regular crRNA	Combined with tracrRNA sequence to form a mock gRNA double-stranded body, the sequence is easily degraded and has a short duration of action.	In vitro and in vivo gene editing with relatively low toxic side effects.
Modified UHP crRNA	Combined with tracrRNA sequences to form a mock gRNA double-stranded body or used alone, it has been modified for high stability, high affinity and relatively high gene editing effect.	In vitro gene editing, or gene targeting for diagnosis.

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