BiOligo can develop and produce different adapter sequences based on customer requirements. These include short adapters, full-length adapters, and specific adapters to meet specific requirements for different contamination rates and library creation standards.
Service details
Service | Specification | Purification | Delivery Standard |
BLG Dual Index | 24/48/96 rxns | DHPLC PAGE+ | • Single tube / 96-hole plate • Dry powder / 15 μM liquid or designated concentration • COA documents |
Advantages
1. Customized synthesis and individual packaging services;
2. Strict quality control to ensure a minimum cross contamination rate, reduced basic group error rate, high purity and accurate quantification of adapter primers;
3. Specific Index sequence to ensure balanced basic adapter groups;
4. Optimized annealing process to ensure the connection efficiency and stability of adapters;
5. Special synthesis processes to guarantee stable phosphorylation modification of the adapters. All batches undergo strict quality control and functional verification.
HPLC purity
The HPLC purity of the adapters exceeds 98.5%, with a CV of 0.29% for 5 nmol adapters containing 69 bp phosphorylation modification and phosphorthioate modification; HPLC purity is 99.01%, with CV of 0.36% for 25 nmol adapters.