Product Details
The BioZues® Heat-labile UNG enzyme catalyzes the hydrolysis of uracil bases in DNA chains, releasing free uracil. This process is commonly used to eliminate cross-contamination from previously amplified products in PCR, qPCR, and RT-qPCR experiments.
Unlike a standard E. coli UNG, the heat-labile UNG is more sensitive to temperature. It is active at room temperature but can be completely inactivated at temperatures above .
Advantages
- Superior Decontamination Efficiency
When a heat-labile UNG at 1 U/reaction was used with uracil-containing amplification products as templates, BiOligo demonstrated the most delayed Ct value compared to other suppliers. This outcome suggests that BiOligo was the most effective at removing contaminant nucleic acids, confirming its superior anti-contamination performance.
- Minimal Impact on Ct Values
Using heat-labile UNG at 1 U/reaction with an RNA template, the ΔCt (Ct with UNG – Ct without UNG) was calculated to assess its effect on RT-qPCR. BiOligo's UNG showed a minimal ΔCt, which suggests it was completely inactivated by heat and had a negligible impact on the Ct values. This performance was comparable to that of Suppliers A and B, but superior to Supplier C.
Component
Downloadable files
SE1102-Heat-labile UNG EN.pdf