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FAQ

• What is the principle behind DNA sequencing? What are the steps? How accurate is ABI 3730XL sequencing?

  The principle of DNA sequencing is the Sanger method (also known as the chain termination method). The specific steps are as follows:

  
  

  1.  Quantification: Quantify the sample and primers.

  
  

  2.  Sequencing Reaction: Add the quantified template and primers to the reaction system along with reagents like BigDye, and perform the sequencing reaction using a PCR machine.

  
  

  3.  Data Reading: Based on the different fluorescent signals, read the base sequence of the tested sample. ABI (Applied Biosystems) currently claims an accuracy of 98.5% for sequencing results within 800 base pairs using the ABI 3730XL sequencing system.