400-168-8906
CN
Modified siRNA

Due to the poor stability of siRNA, it is easily degraded by nucleases in vivo and is not easily absorbed by tissues, which limits its application in vivo. Chemical modification of the synthesized siRNA can increase the stability of siRNA and effectively inhibit the expression of the target gene. The correct modification can greatly promote the transformation of RNAi drugs from in vitro to in vivo and from laboratory to clinical application. The chemical modifications of siRNA mainly include three types: phosphate backbone modification, ribose modification and base modification. In addition, fluorescent dye labeling can also be used to track the absorption and distribution of siRNA in cells or animals.




Modification Choice


Type

Modification

5’

Intel

3’

Fluorescent groups

FAM


Cy3


Cy5


HEX



TET



TAMRA


Quenched groups

BHQ-1



BHQ-2



MGB



Dabcyl



Chemical groups

Biotin


Biotin-teg


C6-NH2



C7-NH2



Thiol


Chol


GalNAc



Special bases

LNA



2’-O-Methyl



2’-MOE



2’-F



5-Me-dC



rl



dl



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Phosphorylation

PHOS


Thiophosphorylation

Phosphorothioate


* The modified RNA synthesis specification is within 5 OD, and the purification method is HPLC purification