Product Details
In real-time PCR workflows, uncalibrated instruments pose hidden risks to data reliability. Changes in optical performance can compromise reproducibility, multiplex accuracy, and detection of weak positives. Regular calibration is therefore essential to maintain stable and reliable instrument performance.
However, in-house calibration is often time-consuming, difficult to standardize, and insufficient for comprehensive optical verification. The BiOligo® Real-Time PCR Systems Spectral Calibration Kit offers a standardized, ready-to-use solution with a defined calibration workflow, enabling fast, consistent, and reliable instrument calibration.
Storage and Shelf Time
Store at -20±5°C, protected from light. Stable for 12 months.
Operating Procedure

Advantages
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Full optical calibration and performance verification workflow, including ROI, background, optical, and dye calibration
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Reliable and consistent fluorescence signals
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Ready-to-use, standardized calibration format
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Platform-independent compatibility with major PCR systems (e.g., ABI 7500, QuantStudio series)
Calibration Performance
Background Calibration

Optical Calibration

Dye Calibration (FAM)

RNase P Verification


Component

