BiOligo offers customized synthesis of mass spectrometry primers for clients based on their specific requirements. Single nucleotide polymorphism (SNP) detection, performed using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF), achieves an accuracy rate of up to 99.9%. With high accuracy, flexibility, high throughput, short detection cycles, and cost-effectiveness, it provides an excellent solution for SNP detection.

Advantages

1. Specialized synthesis and purification processes are employed to achieve primer purity≥95%.

2. Strict control of N+1/N-1 fragment ratio to avoid false positives during detection.

3. Proprietary purification processes are employed to minimize salt impurities in the primer, avoiding the introduction of salt peaks in mass spectrometry results that could interfere with interpretation.

4. Accurate quantification with an error rate ≤ ±10%, avoiding non-specific amplification during multiple PCR processes.

Experimental Procedure

1. PCR amplification of DNA sequences containing the target SNP, followed by purification to remove unbound dNTPs.

2. iPLEX single nucleotide extension: Purified PCR products are used for single nucleotide extension, with a specific extension primer designed for each target SNP. The extension products for each SNP allele differ by only one base at the end.

3. Mass spectrometry detection: Purified extension products are transferred to a SpectroCHIP chip for mass spectrometry analysis. Different alleles of the same SNP are distinguished by separate detection peaks resulted from their distinct molecular weights.