Short Tandem Repeat (STR) primers are DNA sequences composed of 1-6 nucleotide units that are repeated in tandem in the genome. Due to the variation in the number of repeat units between individuals and their abundance, STRs exhibit high levels of polymorphism and are widely present in the genomes of humans and mammals. Currently, fluorescence-labeled detection techniques based on STR analysis are extensively used in genetic mapping, linkage analysis, parentage testing, disease gene localization, and studies of species polymorphism.

The detection of STRs using fluorescence-labeled primers primarily involves multiplex PCR amplification in a reaction system. Multiple pairs of primers labeled with different fluorescent dyes are used for amplification in the same reaction. The resulting PCR products with different fluorescent labels can be distinguished by capillary electrophoresis , as their distinct peak positions. STR probes are primer sequences with a fluorescent group attached to the 5' end. The stability of the fluorescent group significantly affects the interpretation of the detection results. BiOligo has developed an independent synthesis process for STR probes, greatly enhancing the stability of fluorescent labeling.

Advantages

1. Special anti-fall-off synthesis process ensures the stability of fluorescent groups, effectively avoiding interference in result interpretation.

2. Accurate quantification to prevent non-specific amplification.

3. GMP-level production environment to eliminate Anthropogenic pollution.

4. Comprehensive fluorescent group modifications for flexible selection.

5. Multiple purification options, including RP-HPLC, IE-HPLC, DHPLC, etc.