Product Details
The Real-Time PCR Systems Spectral Calibration Kit establishes the pure dye spectra and multicomponent values for FAM, SYBR Green, VIC, JOE, NED, TAMRA, Cy3, ROX, Texas Red, and Cy5 on the 7500 Real-Time PCR System. The kit includes one background plate, one region of interest (ROI) calibration plate, ten spectral calibration plates with ten separate dye standards, and one RNase P verification plate.
Storage and Shelf Time
Store at -20±5°C, protected from light. Stable for 12 months.
Applicable Instruments
Applied Biosystems 7500 Real-Time PCR Systems
Operating Procedure
Component
|
Number |
Component |
Wavelength Range |
Volume |
|
1 |
Region of Interest (ROI) Calibration Plate |
— |
50 μL |
|
2 |
Background Spectral Calibration Plate |
— |
50 μL |
|
3 |
FAM Dye Spectral Calibration Plate |
518±5 nm |
50 μL |
|
4 |
SYBR Green Dye Spectral Calibration Plate |
522±5 nm |
50 μL |
|
5 |
VIC Dye Spectral Calibration Plate |
548±5 nm |
50 μL |
|
6 |
JOE Dye Spectral Calibration Plate |
548±5 nm |
50 μL |
|
7 |
NED Dye Spectral Calibration Plate |
573±5 nm |
50 μL |
|
8 |
CY3 Dye Spectral Calibration Plate |
562±5 nm |
50 μL |
|
9 |
TAMRA Dye Spectral Calibration Plate |
578±5 nm |
50 μL |
|
10 |
ROX Dye Spectral Calibration Plate |
601±5 nm |
50 μL |
|
11 |
Texas Red-X Dye Spectral Calibration Plate |
608±5 nm |
50 μL |
|
12 |
CY5 Dye Spectral Calibration Plate |
670±5 nm |
50 μL |
|
13 |
RNase P Verification Plate |
— |
50 μL |
Downloadable files
AKSTD075-Real-Time PCR Systems Spectral Calibration Kit