Product Details

BioZues® HS Taq U+ Multiple Master Mix (Probe qPCR) is a ready-to-use, 2× concentrated master mix for fluorescent quantitative PCR that offers effective anti-contamination properties.

At its core is BioZues® HS DNA Polymerase, an antibody-blocked Taq DNA Polymerase known for its exceptional stability, high sensitivity, and efficient amplification. The mix also features a unique PCR buffer with optimized concentrations of the hot-start DNA polymerase, UNG enzyme, dUTP Mix (A/G/C/U), and Mg2+. This carefully balanced formulation allows it to easily handle multiple fluorescent quantitative PCR systems.

Advantages

dUTP/UNG anti-pollution system; supports multiple qPCR; ready-to-use.

• Amplification Sensitivity Test

For the amplification sensitivity test, a pUC57 plasmid was serially diluted in TE buffer across seven tenfold gradients. The final, seventh dilution contained approximately 1 copy/µL. Each reaction used 5 µL of this template per well. The results showed that SM0201 has high sensitivity, as it was able to detect as few as 1 copy per reaction.

• Performance Comparison with Competitors

Under identical experimental conditions, BiOligo's SM0201 was tested against products from Supplier A and Supplier B.

Versus Supplier A: The ΔCt was less than 0.5 in the ROX and CY5 channels, and less than 1 in the FAM and VIC channels.

Versus Supplier B: The ΔCt was less than 0.5 in the FAM, ROX, and CY5 channels. However, an abnormal amplification curve was observed in the VIC channel for Supplier B's product.

Component

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